TROUBLESHOOTING FOR MICROTOMY

For technical support, our on-staff histotechnologist is available to answer any questions. Please call 1-888-288-8728.


COMPRESSION

Block too warm: Chill
Room too warm: Cool down
Incorrect angle: Increase
Sectioning too fast: Slow down
Blade dull: Change blade

SPLIT SECTION

Hard particles in block: Soak
Dirty paraffin: Filter or change
Dirty blade: Clean both sides of blade
Incorrect angle: Decrease
Nicks/imperfections in blade edge: Change blade

THICK AND THIN

Something loose on microtome: Tighten screws and blade holder locks
Incorrect angle: Increase
Specimen too large or too hard: Soak
Sectioning too fast: Slow down

VENETIAN BLINDS OR WASHBOARDING

Blade loose in holder: Tighten blade holder or clean holder
Block holder not tight: Tighten blade holder locks
Too large of specimen or too hard: Soak
Incorrect angle: Decrease
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