TROUBLESHOOTING FOR MICROTOMY
For technical support, our on-staff histotechnologist is available to answer any questions. Please call 1-888-288-8728.
COMPRESSION
Block too warm: ChillRoom too warm: Cool down
Incorrect angle: Increase
Sectioning too fast: Slow down
Blade dull: Change blade
SPLIT SECTION
Hard particles in block: SoakDirty paraffin: Filter or change
Dirty blade: Clean both sides of blade
Incorrect angle: Decrease
Nicks/imperfections in blade edge: Change blade
THICK AND THIN
Something loose on microtome: Tighten screws and blade holder locksIncorrect angle: Increase
Specimen too large or too hard: Soak
Sectioning too fast: Slow down
VENETIAN BLINDS OR WASHBOARDING
Blade loose in holder: Tighten blade holder or clean holderBlock holder not tight: Tighten blade holder locks
Too large of specimen or too hard: Soak
Incorrect angle: Decrease